Genetic relatedness analysis of Nocardia strains by random amplification polymorphic DNA: validation and applications
Identifieur interne : 001100 ( France/Analysis ); précédent : 001099; suivant : 001101Genetic relatedness analysis of Nocardia strains by random amplification polymorphic DNA: validation and applications
Auteurs : Frédéric Laurent [France] ; Frédérique Provost [France] ; Andrée Couble [France] ; Emmanuelle Casoli [France] ; Patrick Boiron [France]Source :
- Research in Microbiology [ 0923-2508 ] ; 2000.
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Abstract
Until now, no simple and rapid technique existed for epidemiological study of strains belonging to the Nocardia genus. The application of the arbitrarily primed PCR procedure to generate randomly amplified polymorphic DNA (RAPD) fingerprints for such analysis of Nocardia isolates was investigated. Fifty-one unrelated clinical isolates of N. asteroides were tested. Two conditions of RAPD using two different primers generated RAPD fingerprints that allowed the differentiation of all strains. The patterns were reproducible and discriminating. The results highlight the diversity of N. asteroides species and confirm that RAPD analysis is a highly valuable tool for studying the epidemiology of the Nocardia genus. Several examples describe the advantage of RAPD analysis for establishing the relationship between isolates from a given patient (long-term infections, coinfections) and from different patients (i.e. during an outbreak). In the future, this technique will help us to investigate the source of infection in cases of nosocomial transmission, to understand the outcome of nocardiosis, and to follow the evolution and acquisition of resistance to Nocardia strains.
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DOI: 10.1016/S0923-2508(00)00152-2
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ISTEX:E6E44CC653273BACD40A4DD03A26A2CA12BF36C2Le document en format XML
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The application of the arbitrarily primed PCR procedure to generate randomly amplified polymorphic DNA (RAPD) fingerprints for such analysis of Nocardia isolates was investigated. Fifty-one unrelated clinical isolates of N. asteroides were tested. Two conditions of RAPD using two different primers generated RAPD fingerprints that allowed the differentiation of all strains. The patterns were reproducible and discriminating. The results highlight the diversity of N. asteroides species and confirm that RAPD analysis is a highly valuable tool for studying the epidemiology of the Nocardia genus. Several examples describe the advantage of RAPD analysis for establishing the relationship between isolates from a given patient (long-term infections, coinfections) and from different patients (i.e. during an outbreak). 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